This footage shows part of the aseptic filling stage where peptide solution is manually transferred into vials under sterile airflow.
The process begins with preparation of the peptide solution inside a laminar flow hood. The formulation typically contains the peptide itself, sterile water or buffer, and stabilisers or excipients added to support the formulation during freeze-drying.
Before filling, the solution is passed through a 0.22 micron sterile filter to remove bacteria, microbes, and particulate contamination. This filtration step acts as one of the final sterility barriers before the solution is transferred to the filling system.
The filtered solution is then moved to a second laminar flow workstation where the manual filling takes place. Inside this hood the operator works under HEPA-filtered laminar airflow, which pushes clean air downward across the work surface to reduce contamination while the vials are open.
The filling system uses a peristaltic pump, which moves liquid through sterile tubing without the product contacting the pump mechanism itself. The operator primes the tubing to remove air bubbles before filling begins.
A tray of Type I borosilicate glass vials is placed inside the hood and the tubing outlet is positioned above the tray. During filling, the pump regulates the volume, while the operator guides the dispensing tube across rows of vials so that each vial receives the programmed dose.
Throughout the process the operator visually monitors the liquid level and flow stability to ensure consistent filling.
Although the stoppering stage itself is not shown, the vials appear later with stoppers partially inserted, which protects the vial opening while still allowing vapour to escape during the freeze drying process.
The filled trays are then moved on for the next stage of production, lyophilisation (freeze drying).
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