Janoshik Weighs In On Elora Testing

When The Car Runs Perfectly ; Why Janoshik thinks deeper Elora testing may not be necessary

Krysia

Janoshik Weighs In On Elora Testing

Key Elora testing points from the transcript:

Janoshik did not use a certified reference standard for the first Elora sample.

They ran their usual unknown-peptide workflow instead:

mass spec

HPLC

net peptide content by CHN

NMR outsourced, because their own NMR is not yet running.

The first sample discussed was the Uther sample.

Janoshik said everything “fit perfectly” with what was theoretically expected.

They did not see anything suggesting an issue with the molecule.

His view is that the ring makes Elora interesting, but not necessarily unusually difficult to test by routine methods.

However, he said that if someone wants explicit confirmation that the ring is perfectly correct, that would require much more extensive testing.

He compared it to a car: if the car runs perfectly, you do not normally disassemble it to check the pistons. But if you want to check the pistons, you can, it just takes much more work.

He said deeper confirmation using more advanced NMR/QTOF-style work is possible, but would take a long time and cost thousands.

For routine future Elora samples, they would likely run:

HPLC

quick LC-MS confirmation

Current cost mentioned was around $600 per sample, likely to decrease if testing volume rises.

He said more Elora samples are coming in, including from Uther buyers and at least one other manufacturer.

His overall view: based on their current testing, Elora looked like the correct molecule, and deeper structural confirmation is possible but not necessarily required unless someone specifically wants that level of certainty.

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